Browse dbCAN-PUL Entries

PULID Characterization Method(s) Substrate Organism Publication Publish Date Type Num Genes Num CAZymes CazyFamily
PUL0025 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 32093600
Elucidation of the K32 Capsular Polysaccharide Structure and Characterization of the KL32 Gene Cluster of Acinetobacter baumannii LUH5549. Biochemistry (Mosc). 2020 Feb;85(2):241-247. doi: 10.1134/S000629792002011X.
2020 Feb biosynthesis 18 4 GT2, GT4, GT0, GT2
PUL0046 NMR, sequence homology analysis O-antigen Escherichia coli 29738687
O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104. Biochemistry (Mosc). 2018 May;83(5):534-541. doi: 10.1134/S0006297918050061.
2018 May biosynthesis 16 5 GT2
PUL0047 sugar utilization assay, NMR, sequence homology analysis O-antigen Escherichia albertii 31622726
Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway. Int J Biol Macromol. 2020 Jan 1;142:609-614. doi: 10.1016/j.ijbiomac.2019.09.135. Epub 2019 Oct 14.
2020 Jan 1 biosynthesis 25 8 GT4, GT4
PUL0057 NMR, sequence homology analysis O-antigen Escherichia coli 29787897
Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7.
2018 Jul 15 biosynthesis 13 5 GT4, GT4
PUL0059 NMR, sequence homology analysis O-antigen Escherichia coli 29787897
Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2. Carbohydr Res. 2018 Jul 15;464:8-11. doi: 10.1016/j.carres.2018.05.001. Epub 2018 May 7.
2018 Jul 15 biosynthesis 13 3 GT2
PUL0060 NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 31421354
Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.
2019 Oct 1 biosynthesis 24 4 GT2, GT2, GT4, GT2
PUL0061 NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 31421354
Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.
2019 Oct 1 biosynthesis 35 4 GT2, GT2, GT4, GT2, GT4, CE4
PUL0062 NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 31421354
Acinetobacter baumannii K116 capsular polysaccharide structure is a hybrid of the K14 and revised K37 structures. Carbohydr Res. 2019 Oct 1;484:107774. doi: 10.1016/j.carres.2019.107774. Epub 2019 Aug 8.
2019 Oct 1 biosynthesis 19 4 GT2, GT2, GT4, GT2
PUL0069 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 22 2 GT0, GT2
PUL0070 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 36 3 GT52, GT0, GT14, GT2, GT25, GT4, CE4
PUL0071 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 38 4 GT0, GT14, GT2, GT25, GT4, CE4
PUL0072 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 22 2 GT2, GT0
PUL0073 sugar utilization assay, NMR, sequence homology analysis capsule polysaccharide Acinetobacter baumannii 30664967
Production of the K16 capsular polysaccharide by Acinetobacter baumannii ST25 isolate D4 involves a novel glycosyltransferase encoded in the KL16 gene cluster. Int J Biol Macromol. 2019 May 1;128:101-106. doi: 10.1016/j.ijbiomac.2019.01.080. Epub 2019 Jan 18.
2019 May 1 biosynthesis 22 2 GT2
PUL0090 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia coli 31220629
Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.
2019 Jul 15 biosynthesis 12 4 GT4, GT4, GT4
PUL0130 NMR, mass spectrometry O-antigen Yersinia rohdei 30385338
Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78. Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29.
2019 Feb 1 biosynthesis 11 3 GT4, GT4
PUL0172 gene deletion mutant and growth assay, NMR, sugar utilization assay O-antigen Franconibacter pulveris 27166227
O antigen of FranconibacterpulverisG3872 (O1) is a 4-deoxy-d-arabino-hexose-containing polysaccharide synthesized by the ABC-transporter-dependent pathway. Microbiology (Reading). 2016 Jul;162(7):1103-1113. doi: 10.1099/mic.0.000307. Epub 2016 May 10.
2016 Jul biosynthesis 9 3 GT2, GT2
PUL0173 sequence homology analysis, NMR, sugar utilization assay O-antigen Escherichia coli 27177202
Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal. Carbohydr Res. 2016 Jul 22;430:24-28. doi: 10.1016/j.carres.2016.04.025. Epub 2016 Apr 29.
2016 Jul 22 biosynthesis 20 4 GT4, GT4, GT2
PUL0177 NMR, sugar utilization assay O-antigen Escherichia coli 27293097
Structure and Biosynthesis Gene Cluster of the O-Antigen of Escherichia coli O12. Biochemistry (Mosc). 2016 Apr;81(4):401-6. doi: 10.1134/S0006297916040106.
2016 Apr biosynthesis 15 4 GT4
PUL0181 NMR, sugar utilization assay O-antigen Escherichia coli 27083849
Structure of the beta-l-fucopyranosyl phosphate-containing O-specific polysaccharide of Escherichia coli O84. Int J Biol Macromol. 2016 Jul;88:578-85. doi: 10.1016/j.ijbiomac.2016.04.025. Epub 2016 Apr 13.
2016 Jul biosynthesis 19 5 GT4, GT2
PUL0182 NMR, sugar utilization assay O-antigen Escherichia coli 27058293
Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.
2016 May 13 biosynthesis 12 3 GT2
PUL0183 NMR, sugar utilization assay O-antigen Escherichia coli 27058293
Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.
2016 May 13 biosynthesis 15 3 GT2
PUL0184 NMR, sugar utilization assay O-antigen Escherichia coli 27058293
Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37, and O100. Carbohydr Res. 2016 May 13;426:26-32. doi: 10.1016/j.carres.2016.03.011. Epub 2016 Mar 29.
2016 May 13 biosynthesis 20 3 GT4
PUL0188 NMR, sugar utilization assay O-antigen Escherichia coli 26582605
Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid. Glycobiology. 2016 Apr;26(4):335-42. doi: 10.1093/glycob/cwv106. Epub 2015 Nov 17.
2016 Apr biosynthesis 10 3 GT0, GT2
PUL0198 NMR, sugar utilization assay O-antigen Escherichia coli 26706815
Structure and gene cluster of the o-antigen of Escherichia coli o96. Carbohydr Res. 2016 Feb;420:1-5. doi: 10.1016/j.carres.2015.11.005. Epub 2015 Nov 23.
2016 Feb biosynthesis 13 6 GT2, GT2
PUL0200 NMR, sugar utilization assay O-antigen Escherichia coli 26451883
Structure and genetics of biosynthesis of the glycosyl phosphate-containing O-polysaccharide of Escherichia coli O160. Carbohydr Res. 2015 Nov 19;417:89-93. doi: 10.1016/j.carres.2015.09.007. Epub 2015 Sep 16.
2015 Nov 19 biosynthesis 13 4 GT2, GT2
PUL0201 NMR, sugar utilization assay O-antigen Escherichia coli 26382081
Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170. Carbohydr Res. 2015 Nov 19;417:11-4. doi: 10.1016/j.carres.2015.08.013. Epub 2015 Aug 28.
2015 Nov 19 biosynthesis 13 4 GT2
PUL0202 NMR, sugar utilization assay O-antigen Escherichia coli 26342864
Structure and gene cluster of the O-antigen of Escherichia coli O43. Carbohydr Res. 2015 Oct 30;416:32-6. doi: 10.1016/j.carres.2015.08.008. Epub 2015 Aug 18.
2015 Oct 30 biosynthesis 19 4 GT4, GT2
PUL0219 sugar utilization assay, enzyme activity assay fructooligosaccharide, fructan Lactobacillus paracasei 17644636
Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195. Appl Environ Microbiol. 2007 Sep;73(18):5716-24. doi: 10.1128/AEM.00805-07. Epub 2007 Jul 20.
2007 Sep degradation 7 1 GH32
PUL0286 NMR, mass spectrometry, sugar utilization assay O-antigen Providencia alcalifaciens 23163869
Structural, serological, and genetic characterization of the O-antigen of Providencia alcalifaciens O40. FEMS Immunol Med Microbiol. 2012 Dec;66(3):382-92. doi: 10.1111/1574-695X.12002.
2012 Dec biosynthesis 16 4 GT2, GT4, GT2
PUL0288 acid hydrolysis, NMR, mass spectrometry, sugar utilization assay capsule polysaccharide, heparosan Escherichia coli 22975275
Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res. 2012 Oct 1;360:19-24. doi: 10.1016/j.carres.2012.07.013. Epub 2012 Jul 27.
2012 Oct 1 biosynthesis 5 1 GT2
PUL0290 NMR, mass spectrometry, sugar utilization assay O-antigen Yersinia intermedia 29524727
Full structure and insight into the gene cluster of the O-specific polysaccharide of Yersinia intermedia H9-36/83 (O:17). Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28.
2018 May 2 degradation 12 4 GT4, GT4
PUL0301 sugar utilization assay, NMR O-antigen Escherichia coli 29309918
Structure elucidation of the O-specific polysaccharide by NMR spectroscopy and selective cleavage and genetic characterization of the O-antigen of Escherichia albertii O5. Carbohydr Res. 2018 Mar 2;457:25-31. doi: 10.1016/j.carres.2017.12.010. Epub 2018 Jan 3.
2018 Mar 2 biosynthesis 10 3 GT32, GT2, GT2
PUL0388 sugar utilization assay, NMR O-antigen Salmonella enterica 20501518
Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.
2011 Apr biosynthesis 9 4 GT4, GT4, GT2
PUL0389 sugar utilization assay, NMR O-antigen Escherichia coli 20501518
Structural and genetic characterization of the closely related O-antigens of Escherichia coli O85 and Salmonella enterica O17. Innate Immun. 2011 Apr;17(2):164-73. doi: 10.1177/1753425910369270. Epub 2010 May 25.
2011 Apr biosynthesis 8 4 GT4, GT4, GT2
PUL0431 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia coli 27454490
Structure elucidation and gene cluster characterization of the O-antigen of Escherichia coli O80. Carbohydr Res. 2016 Sep 2;432:83-7. doi: 10.1016/j.carres.2016.07.011. Epub 2016 Jul 14.
2016 Sep 2 biosynthesis 18 4 GT26
PUL0432 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia coli 27645300
Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19.
2017 Feb biosynthesis 11 3 GT4, GT2
PUL0433 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia coli 27645300
Structures and gene clusters of the O-specific polysaccharides of the lipopolysaccharides of Escherichia coli O69 and O146 containing glycolactilic acids: ether conjugates of D-GlcNAc and D-Glc with (R)- and (S)-lactic acid. Glycoconj J. 2017 Feb;34(1):71-84. doi: 10.1007/s10719-016-9730-y. Epub 2016 Sep 19.
2017 Feb biosynthesis 12 3 GT2
PUL0446 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia albertii 28494314
Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5. Carbohydr Res. 2017 Jun 29;446-447:28-31. doi: 10.1016/j.carres.2017.04.024. Epub 2017 May 2.
2017 Jun 29 biosynthesis 17 2 GT4
PUL0447 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia albertii 28672166
Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.
2017 Sep 8 biosynthesis 14 4 GT4, GT4
PUL0448 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia albertii 28672166
Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.
2017 Sep 8 biosynthesis 19 4 GT4
PUL0449 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia albertii 28672166
Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.
2017 Sep 8 biosynthesis 13 4 GT4, GT2, GT4
PUL0450 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia albertii 28672166
Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7. Carbohydr Res. 2017 Sep 8;449:17-22. doi: 10.1016/j.carres.2017.06.008. Epub 2017 Jun 17.
2017 Sep 8 biosynthesis 14 3 GT52, GT2, GT2
PUL0451 sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry O-antigen Streptococcus pneumoniae 28837839
Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.
2017 Oct 10 biosynthesis 18 5 GT4
PUL0452 sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry O-antigen Streptococcus pneumoniae 28837839
Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.
2017 Oct 10 biosynthesis 18 5 GT4, GT2
PUL0453 sequence homology analysis, sugar utilization assay, NMR, gas chromatography and mass spectrometry O-antigen Streptococcus pneumoniae 28837839
Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F. Carbohydr Res. 2017 Oct 10;450:19-29. doi: 10.1016/j.carres.2017.08.006. Epub 2017 Aug 18.
2017 Oct 10 biosynthesis 18 5 GT4
PUL0461 sequence homology analysis, sugar utilization assay, NMR O-antigen Yersinia kristensenii 31220629
Structure elucidation and gene cluster characterization of the O-antigen of Yersinia kristensenii capital ES, Cyrillic-134. Carbohydr Res. 2019 Jul 15;481:9-15. doi: 10.1016/j.carres.2019.06.001. Epub 2019 Jun 6.
2019 Jul 15 biosynthesis 12 4 GT4, GT4, GT4
PUL0462 sequence homology analysis, sugar utilization assay, NMR O-antigen Escherichia coli 24607538
Structure elucidation and gene cluster annotation of the O-antigen of Escherichia coli O39; application of anhydrous trifluoroacetic acid for selective cleavage of glycosidic linkages. Carbohydr Res. 2014 Mar 31;388:30-6. doi: 10.1016/j.carres.2014.02.013. Epub 2014 Feb 18.
2014 Mar 31 biosynthesis 14 4 GT0, GT4, GT2
PUL0486 RT-PCR, sugar utilization assay raffinose Streptococcus pneumoniae 10613841
Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system. Genome Res. 1999 Dec;9(12):1189-97. doi: 10.1101/gr.9.12.1189.
1999 Dec degradation 8 2 GH13, GH13_18, GH36
PUL0582 NMR, microarray, enzyme activity assay, gene deletion mutant and growth assay lactose Lactococcus lactis 22660716
A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363. Appl Environ Microbiol. 2012 Aug;78(16):5612-21. doi: 10.1128/AEM.00455-12. Epub 2012 Jun 1.
2012 Aug degradation 5 1 GH1
PUL0627 sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis capsule polysaccharide Acinetobacter baumannii LUH5543 33159946
Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4.
2021 Jan 1 biosynthesis 21 1 GT2
PUL0628 sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis capsule polysaccharide Acinetobacter baumannii BAL_204 33159946
Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4.
2021 Jan 1 biosynthesis 22 2 GT2, GT2, GT2, GT2
PUL0629 sugar utilization assay, NMR, Smith degradation, mass spectrometry, sequence homology analysis capsule polysaccharide Acinetobacter baumannii BAL_309 33159946
Involvement of a multifunctional rhamnosyltransferase in the synthesis of three related Acinetobacter baumannii capsular polysaccharides, K55, K74 and K85. Int J Biol Macromol. 2021 Jan 1;166:1230-1237. doi: 10.1016/j.ijbiomac.2020.11.005. Epub 2020 Nov 4.
2021 Jan 1 biosynthesis 22 1 GT2, GT2
PUL0648 high performance anion exchange chromatography, substrate binding assay, thin layer chromatography, NMR, mass spectrometry, crystallization glucuronoarabinoxylan Dysgonomonas mossii DSM 22836 33667545
A polysaccharide utilization locus from the gut bacterium Dysgonomonas mossii encodes functionally distinct carbohydrate esterases. J Biol Chem. 2021 Jan-Jun;296:100500. doi: 10.1016/j.jbc.2021.100500. Epub 2021 Mar 2.
2021 Jan-Jun degradation 37 20 CBM0, 3.2.1.8, CBM4, GH10, GH43_12, GH43, GH43, GH43_1, GH10, GH146, CBM48, CE1, CBM6, CBM36, GH8, 3.2.1.156, CE6, CBM2, GH43_29, GH43, CBM22, CBM42, CBM66, CBM6, CBM6, GH43_29, GH43, GH97, GH31, GH51, CBM2, GH43_29, GH43, CBM22, CBM42, CBM6, CBM48, CE1, GH10, GH43_10, GH43, GH115, GH10, GH43, GH43_1, GH67
PUL0649 Smith degradation, NMR capsule polysaccharide Acinetobacter baumannii B8300 33667610
A novel ItrA4 d-galactosyl 1-phosphate transferase is predicted to initiate synthesis of an amino sugar-lacking K92 capsular polysaccharide of Acinetobacter baumannii B8300. Res Microbiol. 2021 Apr-May;172(3):103815. doi: 10.1016/j.resmic.2021.103815. Epub 2021 Mar 3.
2021 Apr-May biosynthesis 22 1 GT2, GT2
PUL0650 enzyme activity assay, high performance anion exchange chromatography, recombinant protein expression, NMR, gene deletion mutant and growth assay arabinogalactan Bifidobacterium longum JCM 7052 33674431
Novel 3-O-alpha-d-Galactosyl-alpha-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum. Appl Environ Microbiol. 2021 Apr 27;87(10):e02690-20. doi: 10.1128/AEM.02690-20. Print 2021 Apr 27.
2021 Apr 27 degradation 7 2 GH36, GH39, CBM35
PUL0651 enzyme activity assay, NMR agar Gilvimarinus chinensis DSM 19667 33691998
Agarase cocktail from agar polysaccharide utilization loci converts homogenized Gelidium amansii into neoagarooligosaccharides. Food Chem. 2021 Aug 1;352:128685. doi: 10.1016/j.foodchem.2020.128685. Epub 2020 Nov 19.
2021 Aug 1 degradation 63 14 CBM6, GH16_16, GH16, GH50, CBM6, CBM13, CBM6, GH16_16, GH16, GH50, GH117, GH16_3, GH16, GH2, GH50, GH127, GH167, CBM2, CBM6, GH86, 3.2.1.81, GH50, 3.2.1.81, GH86
PUL0657 recombinant protein expression, NMR levoglucosan  Bacillus smithii S-2701M 33208778
Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction. Sci Rep. 2020 Nov 18;10(1):20066. doi: 10.1038/s41598-020-77133-8.
2020 Nov 18 degradation 8 2 GH109, GH109
PUL0660 NMR, Smith degradation capsule polysaccharide Acinetobacter baumannii 48-1789 34073255
Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641.
2021 May 26 biosynthesis 21 2 GT2, GT4
PUL0661 NMR, Smith degradation capsule polysaccharide Acinetobacter baumannii MAR-24 34073255
Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides. Int J Mol Sci. 2021 May 26;22(11):5641. doi: 10.3390/ijms22115641.
2021 May 26 biosynthesis 22 2 GT2, GT4
PUL0670 NMR, Smith degradation, mass spectrometry capsule polysaccharide Acinetobacter baumannii KZ-1098 34537298
The K26 capsular polysaccharide from Acinetobacter baumannii KZ-1098: Structure and cleavage by a specific phage depolymerase. Int J Biol Macromol. 2021 Nov 30;191:182-191. doi: 10.1016/j.ijbiomac.2021.09.073. Epub 2021 Sep 16.
2021 Nov 30 biosynthesis 24 2 GT2, GT4
PUL0672 Smith degradation, NMR, mass spectrometry capsule polysaccharide Acinetobacter baumannii Ab-46-1632 34757131
Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis. Int J Biol Macromol. 2021 Dec 15;193(Pt B):1294-1300. doi: 10.1016/j.ijbiomac.2021.10.178. Epub 2021 Oct 30.
2021 Dec 15 biosynthesis 20 4 GT2, GT2, GT2, GT2
PUL0673 NMR, substrate binding assay, liquid chromatography and mass spectrometry human milk oligosaccharide Bifidobacterium pseudocatenulatum DSM20438 34757822
Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl Environ Microbiol. 2022 Jan 25;88(2):e0170721. doi: 10.1128/AEM.01707-21. Epub 2021 Nov 10.
2022 Jan 25 degradation 8 1 GH95